Anti-LRAT Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O95237

Core Product Specifications and Parameters

Biological Background: LRAT Function and Localization

• LRAT (Lecithin retinol acyltransferase, also known as phosphatidylcholine--retinol O-acyltransferase) is a 25.7 kDa membrane-associated enzyme that transfers an acyl group from phosphatidylcholine to all-trans retinol, yielding all-trans retinyl esters, the primary storage form of vitamin A. Related references: PMID:9920938
• These retinyl esters are essential for vision, serving as substrates for the isomerohydrolase in the retinal pigment epithelium, which generates 11-cis-retinol that is subsequently oxidized to 11-cis-retinaldehyde—the chromophore for rhodopsin and cone photopigments.
• LRAT is required for the survival of cone photoreceptors and the maintenance of normal rod photoreceptor cell morphology.
• The protein localizes primarily to the endoplasmic reticulum membrane and rough ER, and is also detected in endosomes, multivesicular bodies, and the cytoplasmic perinuclear region.
• At the protein level, LRAT is highly expressed in testis and liver, with significant levels also in the retinal pigment epithelium, small intestine, prostate, pancreas, and colon; low expression is observed in the brain. In fetal tissues, it is found in retinal pigment epithelium and liver, but barely in the brain.
• Clinically, LRAT mutations are linked to Leber congenital amaurosis (LCA), a severe early-onset retinal dystrophy, highlighting its critical role in vision.

Experimental Guidance and Technical Tips

• For Western blotting, consider using a reducing gel and optimize transfer conditions, as LRAT is a transmembrane protein with a molecular weight of approximately 26 kDa.
• In IF/ICC experiments, fixation and permeabilization protocols should be carefully titrated to preserve endoplasmic reticulum membrane integrity, where LRAT predominantly resides.
• Given that the antibody is a rabbit polyclonal, it may recognize multiple epitopes; validate specificity in LRAT-relevant tissues (e.g., liver, testis) using appropriate controls.

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