Anti-LPCAT4 Polyclonal Antibody for WB, ELISA - Q643R3

Core Product Specifications and Parameters

Biological Background: LPCAT4 Function and Localization

• LPCAT4, also known as AGPAT7, AYTL3, or LPEAT2, is a lysophospholipid acyltransferase that belongs to the 1-acylglycerol-3-phosphate O-acyltransferase family. It catalyzes the conversion of various lysophospholipids to their respective phospholipids using acyl-CoA as an acyl donor.
• The enzyme displays broad substrate specificity: it acts on lysophosphatidylethanolamine, lysophosphatidylcholine, 1-alkenyl-lysophosphatidylethanolamine, lysophosphatidylglycerol, and alkyl-lysophosphatidylcholine, but not on lysophosphatidylinositol, glycerol-3-phosphate, diacylglycerol, or lysophosphatidic acid.
• LPCAT4 preferentially utilizes long-chain acyl-CoAs (C16 and C18) as acyl donors, playing a key role in phospholipid remodeling and membrane biogenesis.
• The protein localizes to the endoplasmic reticulum membrane, consistent with its function in phospholipid metabolism.
• LPCAT4 is ubiquitously expressed with highest levels in the brain, suggesting an important role in neural tissue phospholipid homeostasis.
• It is a glycoprotein and a multi-pass transmembrane protein, indicative of its integral membrane localization and post-translational modifications.
• As a transferase involved in lipid biosynthesis and metabolism, LPCAT4 is critical for maintaining cellular membrane structure and function.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 345-524 of human LPCAT4, which covers the C-terminal region. This region is predicted to be exposed on the cytoplasmic side of the endoplasmic reticulum membrane, potentially facilitating antibody recognition in applications such as WB.
• For Western blot, consider using lysates from cell lines or tissues known to express LPCAT4 (e.g., brain, liver). The predicted molecular weight is approximately 57 kDa; however, post-translational modifications such as glycosylation may affect migration. Validate the band size using appropriate positive and negative controls.
• ELISA performance can be optimized by testing different coating antigen concentrations and antibody dilutions. Since this is a polyclonal antibody, batch-to-batch consistency should be verified for quantitative assays.
• Cross-reactivity with mouse is indicated, enabling use in rodent model systems. Consider confirming reactivity in your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This LPCAT4 rabbit polyclonal antibody supports research into lipid metabolism, membrane biology, and related disorders. As a trusted partner, CamelBio also provides technical services and consulting to accelerate your project from concept to clinic, with a commitment to sourcing even rare-target raw materials efficiently.