Anti-LPCAT1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8NF37

Core Product Specifications and Parameters

Biological Background: LPCAT1 Function and Localization

• LPCAT1 encodes lysophosphatidylcholine acyltransferase 1, also referred to as AYTL2, PFAAP3, and AGPAT9, indicating its classification within the 1-acylglycerol-3-phosphate O-acyltransferase family.
• It catalyzes the conversion of lysophosphatidylcholine (LPC) to phosphatidylcholine (PC), a pivotal reaction in phospholipid metabolism and pulmonary surfactant biosynthesis. Related references: PMID:18156367 PMID:21498505
• The enzyme exhibits acetyltransferase activity, converting lyso-platelet-activating factor (lyso-PAF) into platelet-activating factor (PAF), thereby playing a role in inflammatory and thrombotic processes.
• LPCAT1 localizes to the endoplasmic reticulum membrane, Golgi apparatus membrane, cell membrane, and lipid droplets, reflecting its multifaceted roles in lipid trafficking and organelle dynamics.
• Its activity is calcium-independent, and it displays a preference for saturated fatty acyl-CoAs, containing metal-binding motifs that may contribute to its enzymatic mechanism.
• Expression is notably high in erythrocytes, suggesting specialized functions in red blood cell lipid homeostasis.
• LPCAT1 is implicated in regulating lipid droplet number and size, further underscoring its importance in cellular lipid storage and metabolism. Related references: PMID:25491198

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (residues 325–534) of human LPCAT1, which may recognize full-length and truncated isoforms; a band near 59 kDa is anticipated in Western blot.
• For Western blot, consider using reducing conditions and whole-cell lysates from human, mouse, or rat tissues known to express LPCAT1, such as lung or erythrocyte-rich samples.
• In immunofluorescence/immunocytochemistry, detection of membrane and organelle-associated signals may require permeabilization; verify localization patterns against known subcellular markers.
• ELISA applications may benefit from optimizing coating antigen and antibody dilutions based on the specific assay format; cross-reactivity with mouse and rat orthologs should be confirmed in the relevant sample matrices.
• As with any polyclonal antibody, lot-to-lot variability is possible; include appropriate positive and negative controls (e.g., knockdown or knockout cells) to validate specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to a comprehensive range of IVD raw materials, technical services, and consulting—from concept to clinic. This anti-LPCAT1 polyclonal antibody supports lipid metabolism and respiratory disease research, complementing our portfolio of validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents. For projects requiring rare-target sourcing or custom assay development, our global supply network is ready to assist with tailored solutions.