Anti-LPAR4 Polyclonal Antibody for WB, ELISA - Q99677

Core Product Specifications and Parameters

Biological Background: LPAR4 Function and Localization

• Lysophosphatidic acid receptor 4 (LPAR4), also known as GPR23, P2Y9, or LPA4, is a member of the G protein-coupled receptor (GPCR) family that binds the bioactive lipid mediator lysophosphatidic acid (LPA).
• Upon activation by LPA, LPAR4 transduces intracellular signals by increasing cytosolic calcium levels and stimulating adenylyl cyclase activity, with a potency rank order of 1-oleoyl-LPA > 1-stearoyl- > 1-palmitoyl- > 1-myristoyl- > 1-alkyl- > 1-alkenyl-LPA.
• The receptor is encoded by the LPAR4 gene (GeneID: 2846) and belongs to the purinergic receptor family, sharing structural and functional features with other lipid-binding GPCRs.
• LPAR4 is localized primarily at the cell membrane, consistent with its role as a transmembrane receptor mediating extracellular LPA signaling.
• Tissue expression studies reveal high levels of LPAR4 in the ovary, while it is undetectable in several brain regions including thalamus, putamen, caudate, frontal cortex, pons, hypothalamus, and hippocampus.
• The protein contains key structural features such as disulfide bonds, N-glycosylation sites, and seven transmembrane helices, and is identified as a proteomics target.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a cytoplasmic C-terminal region (amino acids 311-370) of human LPAR4, which is highly conserved; this design may support reactivity across human, mouse, and rat, as suggested by the reported cross-reactivity.
• For Western blotting, expect a band near 42 kDa; consider using whole-cell or membrane-enriched lysates from tissues such as ovary where LPAR4 expression is high to verify the band.
• When performing ELISA, the antibody can be evaluated for direct antigen binding against recombinant LPAR4 protein or peptides; optimization of coating conditions and detection steps is recommended.
• As a polyclonal antibody, lot-to-lot consistency should be monitored, and appropriate positive and negative controls should be included in every experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material procurement, providing a seamless bridge from research to clinical application. This anti-LPAR4 polyclonal antibody exemplifies our commitment to supplying high-quality reagents for GPCR and cell signaling studies. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and custom sourcing for rare or hard-to-find targets, all supported by expert technical consultation to accelerate your assay development.