Anti-LOXL2 Polyclonal Antibody for WB, ELISA - Q9Y4K0

Core Product Specifications and Parameters

Biological Background: LOXL2 Function and Localization

• LOXL2 (Lysyl oxidase homolog 2) is a copper-dependent amine oxidase that mediates oxidative deamination of lysine residues. It acts as a transcription corepressor by specifically deaminating trimethylated 'Lys-4' of histone H3 (H3K4me3) and also deaminates TAF10 to release it from promoters, inhibiting TFIID-dependent transcription. Related references: PMID:27735137 PMID:25959397
• It shows strict substrate specificity, active only on H3K4me3 and not on H3K9me3, H3K27me3, or partially methylated H3K4, highlighting its precise role in epigenetic transcriptional regulation. Related references: PMID:27735137
• LOXL2 is a key mediator of epithelial-to-mesenchymal transition (EMT). It interacts with SNAI1 to repress E-cadherin (CDH1) expression, likely through histone H3 deamination. LOXL2 also binds endoplasmic reticulum HSPA5, activating the IRE1-XBP1 pathway to drive EMT. Related references: PMID:16096638 PMID:24414204 PMID:27735137 PMID:28332555
• Under hypoxic conditions, LOXL2 represses E-cadherin, contributing to tumor aggressiveness. It is frequently upregulated in various cancers, suggesting a role in tumor progression. Related references: PMID:20026874
• Secreted LOXL2 functions in the extracellular matrix, crosslinking collagen and elastin through oxidative deamination of peptidyl lysines. It also regulates sprouting angiogenesis via collagen IV scaffolding. Related references: PMID:20306300 PMID:21835952
• The enzyme contains a lysyl tyrosylquinone (LTQ) cofactor essential for its catalytic activity, formed by post-translational crosslinking of lysine and tyrosine residues. It also binds copper and features multiple disulfide bonds and glycosylation sites.
• Subcellular localization is dual: LOXL2 is secreted to the extracellular matrix and basement membrane but also resides in the nucleus, chromosomes, and endoplasmic reticulum, consistent with its transcriptional regulatory and extracellular functions.
• LOXL2 is broadly expressed, with highest levels in reproductive tissues (placenta, uterus, prostate). Expression is elevated in esophageal epithelium layers and numerous carcinomas, making it a potential biomarker. Related references: PMID:10212285 PMID:22204712

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 51–290, encompassing part of the LOX catalytic domain. This region may detect both full-length and truncated LOXL2 isoforms; validate antibody specificity in your target system.
• For Western blot (WB), prepare lysates with protease/phosphatase inhibitors, and use a loading control compatible with the expected 87 kDa band. Confirm reactivity with human, mouse, and rat samples as listed.
• In ELISA applications, consider pairing with a detection antibody that recognizes a non-overlapping LOXL2 epitope for sandwich assays. Optimize coating, blocking, and detection conditions empirically.
• Because LOXL2 localizes to both nuclear and secreted compartments, select lysis buffers and fractionation methods appropriate for your sample type to ensure target recovery.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, serving diagnostic manufacturers and research laboratories with end-to-end support from discovery to clinical application. For LOXL2 research—spanning cancer, fibrosis, EMT, and epigenetic regulation—we provide validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Leverage our expertise to source even rare-target raw materials efficiently, accelerating your assay development.