Anti-LOX Polyclonal Antibody for WB - P28300

Core Product Specifications and Parameters

Biological Background: LOX Function and Localization

• LOX is a copper-dependent amine oxidase that catalyzes the oxidative deamination of peptidyl lysine residues in collagen and elastin precursors, essential for the formation of covalent cross-links in the extracellular matrix (PubMed:26838787). Related references: PMID:26838787
• It functions as a regulator of Ras expression and may act as a tumor suppressor, playing a role in suppressing Ras-driven oncogenesis.
• LOX contributes to aortic wall architecture and integrity, with defects associated with aortic aneurysm (By similarity).
• The protein is secreted and localizes to the extracellular space, consistent with its role in extracellular matrix remodeling.
• LOX is highly expressed in heart, placenta, skeletal muscle, kidney, lung, and pancreas.
• The enzyme contains a lysine tyrosylquinone (LTQ) cofactor and requires copper for catalytic activity; it undergoes post-translational modifications including glycosylation, disulfide bond formation, and sulfation.

Experimental Guidance and Technical Tips

• The immunogen encompasses residues 300-400 within the LOX C-terminal domain. For optimal results, consider diluting to 1:500-1:2000 for Western blot and titrating for other applications.
• For IHC-P and IF/ICC, perform antigen retrieval if detecting endogenous LOX in formalin-fixed paraffin-embedded tissues; validate in relevant tissue sections with known expression.
• LOX is secreted; ensure that protocols for secretion-blocking agents (e.g., Brefeldin A) are considered when assessing intracellular staining.
• The antibody recognizes human, mouse, and rat LOX; cross-reactivity with other species should be confirmed experimentally.
• Avoid using harsh stripping buffers for membrane re-probing in WB, as the signal may be affected by the protein's disulfide bonds.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. This anti-LOX antibody supports extracellular matrix and oncology research, and our team can source complementary reagents to accelerate your diagnostic development pipeline. Connect with us for customized solutions from concept to clinic.