Anti-Loricrin Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - P23490

Core Product Specifications and Parameters

Biological Background: Loricrin Function and Localization

• Loricrin (also known as LOR or LRN) is a major protein component of the keratinocyte cell envelope, providing structural integrity to the epidermis.
• It is predominantly expressed in terminally differentiated keratinocytes of the epidermis and other stratified squamous epithelia.
• The protein localizes to both the cytoplasm and the nucleus, with a notable presence in the nucleoplasm, suggesting dynamic roles in cellular architecture.
• Loricrin is rich in cysteine and glycine residues, forming disulfide bonds and isopeptide cross-links that contribute to the mechanical resilience of the cornified envelope.
• Genetically, the human LORICRIN gene (Gene ID 4014) encodes a protein with a predicted molecular weight of approximately 25.8 kDa (SWISS P23490), though it may migrate at ~26 kDa in SDS-PAGE.
• Mutations in LORICRIN are associated with skin disorders such as ichthyosis and palmoplantar keratoderma, underscoring its essential role in epidermal barrier function.

Experimental Guidance and Technical Tips

• When performing Western blotting, expect a band near 26 kDa; pre-stained markers in that range can help confirm protein weight.
• For immunofluorescence (IF/ICC), permeabilization with 0.1–0.5% Triton X-100 is recommended to access both cytoplasmic and nuclear pools of loricrin.
• The immunogen targets the N-terminal region (aa 1–100), so this antibody may recognize full-length loricrin; validate specificity in knockout or siRNA-treated samples if needed.
• This antibody is predicted to cross-react with mouse and rat loricrin based on sequence homology; verify reactivity in your specific tissue lysates.
• For ELISA, consider using a coating buffer at pH 9.6 and titrating the antibody to determine optimal dilution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, streamlining your development from concept to clinic. This anti-Loricrin polyclonal antibody supports dermatological research and diagnostic assay development, complementing our portfolio of validated antibody pairs, optimized monoclonals, and bulk ancillary reagents. Contact our team for personalized technical support and scalable procurement options.