Anti-LONP2 Rabbit Polyclonal Antibody for WB, ELISA - Q86WA8

Core Product Specifications and Parameters

Biological Background: LONP2 Function and Localization

• LONP2 (Lon protease homolog 2, peroxisomal) is also known as Lon protease-like protein 2 (Lon protease 2) and peroxisomal Lon protease.
• It is an ATP-dependent serine protease that mediates selective degradation of misfolded and unassembled polypeptides in the peroxisomal matrix (By similarity).
• LONP2 is required for the processing of type 2 peroxisome targeting signal (PTS2)-containing proteins and facilitates peroxisome matrix protein import.
• It may indirectly regulate peroxisomal fatty acid β-oxidation by degrading self-processed forms of TYSND1.
• The protein localizes to the peroxisome matrix.
• It is widely expressed, with highest expression in liver, kidney, and pancreas.
• Post-translational modifications include acetylation; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blotting, use whole-cell lysates from human, mouse, or rat tissues (e.g., liver, kidney) or cell lines; note the predicted molecular weight of ~95 kDa (precursor) and consider possible alternatives due to processing.
• Optimize antibody dilution empirically; a starting range of 1:500–1:2000 for WB and 1:500–1:5000 for ELISA is commonly used for polyclonal antibodies.
• Validate detection in peroxisome-enriched fractions if specific subcellular localization is of interest.
• Cross-reactivity with mouse and rat enables use in rodent models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials. For LONP2, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for peroxisomal biology and metabolic disorder research. Our sourcing network can also help locate rare target raw materials to accelerate your assay development.