Anti-LNX1 Rabbit Polyclonal Antibody for WB, ELISA - Q8TBB1

Core Product Specifications and Parameters

Biological Background: LNX1 Function and Localization

• LNX1 (Ligand of Numb-protein X 1) is an E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NUMB isoforms p66 and p72.
• It acts as a RING-type E3 ubiquitin transferase, accepting ubiquitin from E2 enzymes and directly transferring it to targeted substrates.
• Isoform 2 provides an endocytic scaffold for IGSF5/JAM4, suggesting a role in endocytic trafficking pathways.
• The protein localizes to the cytoplasm.
• Expression is detected in heart, placenta, kidney, pancreas, and brain.
• LNX1 contains PDZ domains and a RING finger domain, facilitating protein-protein interactions and ubiquitin ligase activity.
• Post-translational modifications include phosphorylation (phosphoprotein) and zinc ion binding (metal-binding, zinc-finger), with alternative splicing producing multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 501-594 of human LNX1 (NP_001119800.1); verify that this region is present in your target isoform(s) and species of interest, as alternative splicing may affect detection.
• For Western blot, consider using whole-cell lysates from tissues or cell lines known to express LNX1, such as heart, placenta, kidney, pancreas, or brain. The predicted molecular weight is 81 kDa, but isoforms may exhibit variation.
• For ELISA, ensure the recombinant protein immunogen is appropriately coated and test antibody binding in direct or sandwich format as needed.
• Validate cross-reactivity with mouse and rat samples experimentally, as sequence homology in the immunogen region may influence detection sensitivity.
• Because LNX1 is a cytoplasmic E3 ligase, fractionation or enrichment strategies may be considered if endogenous expression levels are low.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers targeting ubiquitin ligases like LNX1, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support in sourcing rare-target raw materials to accelerate your assay development.