Anti-LMX1B Rabbit Polyclonal Antibody for WB, ELISA - O60663

Core Product Specifications and Parameters

Biological Background: LMX1B Function and Localization

• LMX1B is a LIM homeobox transcription factor that critically regulates podocyte-expressed genes, contributing to kidney filtration barrier maintenance. Related references: PMID:24042019 PMID:28059119
• It is essential for dorsal limb fate specification during embryonic development, particularly at the zeugopod and autopod levels.
• The protein localizes predominantly to the nucleus, consistent with its role as a transcription factor.
• LMX1B contains two N-terminal LIM domains that mediate protein-protein interactions and a central homeobox DNA-binding domain.
• Alternative splicing generates multiple isoforms, although the full-length isoform is primarily characterized.
• It is classified under developmental proteins and is associated with disease variants, notably in nail-patella syndrome (NPS).
• Tissue expression is broad, with highest levels in testis, thyroid, duodenum, skeletal muscle, and pancreatic islets.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 136–395 of human LMX1B, a region that may include conserved functional domains; consider sequence alignment to assess suitability for rat cross-reactivity.
• For Western blot, an expected band near 45 kDa should be observed; optimize loading and detection conditions for your specific sample type.
• In ELISA applications, titrate the antibody to determine optimal coating and detection concentrations.
• As a polyclonal antibody, lot-to-lot consistency may vary; validate each new lot in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials—from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents and rare-target sourcing. This LMX1B antibody supports projects in podocyte biology, limb development, and related transcriptional regulation studies, and CamelBio can further assist with customized procurement or technical consultation for your diagnostic pipeline.