Anti-LMX1B Polyclonal Antibody for WB, ELISA - O60663

Core Product Specifications and Parameters

Biological Background: LMX1B Function and Localization

• LMX1B is a LIM homeobox transcription factor (also known as LMX-1.2) that regulates gene expression in podocytes, impacting kidney filtration function. Related references: PMID:24042019 PMID:28059119
• Essential for dorsal limb fate specification at both zeugopodal and autopodal levels during embryonic development.
• Contains a homeobox DNA-binding domain and two N-terminal LIM zinc-binding domains, classifying it as a transcriptional regulator.
• Localizes to the nucleus, consistent with its role in transcription regulation.
• Widely expressed across tissues; highest levels in testis, thyroid, duodenum, skeletal muscle, and pancreatic islets.
• Alternative splicing generates multiple protein isoforms, potentially modulating its activity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (amino acids 1–100). Consider that the antibody may recognize multiple isoforms if they share this epitope.
• For Western blot, a band around 45 kDa is expected. Validate detection in relevant species (human, mouse, rat) with appropriate positive and negative controls.
• In ELISA, use the peptide or full-length protein as coating antigen; titrate antibody concentration for optimal signal-to-noise ratio.
• Because LMX1B localizes to the nucleus, ensure proper nuclear protein extraction for consistent detection in tissue or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including this LMX1B polyclonal antibody for podocyte biology and developmental research. Beyond ready-to-use antibodies, we provide validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and targeted sourcing for rare targets—helping you streamline development from concept to clinic.