Anti-LMOD1 Polyclonal Antibody for WB, IHC-P, ELISA - P29536

Core Product Specifications and Parameters

Biological Background: Leiomodin-1 Function and Localization

• Leiomodin-1 (LMOD1), also known as 64 kDa autoantigen 1D or smooth muscle leiomodin, is an actin-binding protein encoded by the LMOD1 gene and belongs to the tropomodulin family.
• It mediates nucleation of actin filaments and is required for proper contractility of visceral smooth muscle cells. Related references: PMID:28292896
• LMOD1 localizes to the cytoplasm, specifically associated with myofibrils, sarcomeres, and the cytoskeleton in muscle cells.
• Tissue specificity studies show detection in lung vascular smooth muscle, thyroid, extraocular smooth muscle, heart, aorta, skeletal muscle, colon, urinary bladder, uterus, stomach, and small intestine. Related references: PMID:27144530, PMID:2026759, PMID:11318603
• The protein is associated with alternative splicing and phosphorylation, and recognized as a thyroid-associated ophthalmopathy autoantigen.
• Mutations in LMOD1 have been linked to disease variants such as megacystis-microcolon-intestinal hypoperistalsis syndrome (MMIHS), highlighting its role in smooth muscle function.

Experimental Guidance and Technical Tips

• For WB, the predicted molecular weight is 67 kDa; however, alternative splicing may produce additional bands. Validate with appropriate positive controls (e.g., smooth muscle lysates).
• IHC-P detection may require antigen retrieval optimization and careful selection of tissue sections rich in smooth muscle (e.g., colon, bladder).
• For ELISA, consider using the immunogen sequence (aa 29-187) as coating antigen to assess antibody specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for leiomodin-1 research. Our team also supports rare target raw-material sourcing, ensuring reliable supply for smooth muscle biology and autoimmune disease applications.