Anti-LMO4 Rabbit Polyclonal Antibody for IF/ICC and ELISA - P61968

Core Product Specifications and Parameters

Biological Background: LMO4 Function and Localization

• LMO4 (LIM domain only protein 4) is a LIM domain transcription cofactor, also known as breast tumor autoantigen.
• It plays a role in establishing motor neuron identity by cooperating with MNX1 to disrupt LDB1-LHX3 complexes, thereby negatively modulating interneuron genes.
• Contains LIM domains, which are cysteine-rich zinc-binding motifs involved in protein-protein interactions and transcriptional regulation.
• Classified under the LIM-only (LMO) protein family, which function as adaptors in multiprotein complexes.
• Keywords include transcription regulation, metal-binding, zinc, and repeat (due to tandem LIM domains).
• The human LMO4 gene encodes a 17994 Da protein (calculated molecular weight), with observed band around 18 kDa.
• Although subcellular localization is not specified, LIM domain proteins often shuttle between nucleus and cytoplasm.

Experimental Guidance and Technical Tips

• For IF/ICC, consider using cell lines or primary cells expressing LMO4, such as neuronal or breast cancer cells.
• Since the immunogen spans near full-length LMO4, the antibody likely recognizes endogenous levels; validate expression in your model system.
• ELISA applications may require optimization of coating antigen and antibody dilution; consult standard protocols.
• Due to reactivity with mouse and rat, this antibody is suitable for cross-species studies, but confirm with appropriate controls.
• If performing Western blot, a band near the predicted 18 kDa is expected; however, this application is not yet validated by the supplier.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials and technical support. This anti-LMO4 polyclonal antibody supports studies in transcription regulation and neuronal development, and we offer bulk supply, custom antibody services, and complementary reagents to accelerate your assay development. From concept to clinic, our sourcing bridge simplifies procurement for rare targets like LMO4.