Anti-LMO4 Rabbit Monoclonal Antibody for WB and ELISA - P61968

Core Product Specifications and Parameters

Biological Background: LMO4 Function and Localization

• LMO4 (LIM domain only protein 4) is a LIM domain transcription factor and cofactor, also known as breast tumor autoantigen.
• It functions as a transcription cofactor and plays a critical role in motor neuron identity determination, collaborating with MNX1.
• Mechanistically, LMO4 disrupts LDB1-LHX3 complexes, thereby negatively modulating interneuron gene expression in motor neurons.
• Contains LIM domains, which are zinc-binding motifs involved in protein-protein interactions and transcriptional regulation.
• Keywords from UniProt: 3D-structure, LIM domain, metal-binding, proteomics identification, reference proteome, repeat, transcription regulation, zinc.
• Molecular weight predicted at ~18 kDa (17994 Da).
• As a transcription regulator, LMO4 is involved in developmental processes and may have implications in carcinogenesis as a breast tumor autoantigen.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 66-165 of human LMO4 (P61968). For Western blot, consider using total cell lysates from human, mouse, or rat tissues/cell lines; a band at approximately 18 kDa is expected under reducing conditions.
• This antibody is recommended for WB and ELISA; for other applications like IHC or IF, validation in the relevant sample system is required.
• Since LMO4 is a transcription factor, it may have low abundance; consider optimizing loading amounts and detection sensitivity.
• Cross-reactivity with mouse and rat is indicated; however, always verify species-specific reactivity and background in the experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw material partner, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers studying transcription regulation and motor neuron development, we provide reliable access to hard-to-find targets like LMO4, along with tailored technical support from concept to clinic.