Anti-LMO2 Monoclonal Antibody for WB, ELISA - P25791

Core Product Specifications and Parameters

Biological Background: LMO2 Function and Localization

• LMO2 encodes Rhombotin-2, a LIM domain-containing protein (P25791).
• It functions as a transcriptional regulator critical for erythroid development, acting in complexes with TAL1/SCL and LDB1.
• LMO2 is a nuclear protein localized predominantly in the nucleus.
• The protein contains two LIM domain repeats, which serve as zinc-binding motifs mediating protein-protein interactions.
• LMO2 is a known proto-oncogene; chromosomal rearrangements involving LMO2 are associated with T-cell acute lymphoblastic leukemia.
• Alternative splicing generates multiple isoforms of LMO2, potentially contributing to functional diversity.
• Metal-binding properties (zinc) are intrinsic to its LIM domains and structural integrity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human LMO2; verify antibody specificity in relevant human cell or tissue models.
• For western blotting, the predicted molecular weight is 18 kDa; use positive controls such as erythroleukemia cell lines (e.g., K562, HEL) to confirm band detection.
• In ELISA applications, consider using recombinant LMO2 protein to establish standard curves and assess binding affinity.
• Since cross-reactivity is limited to human, validate the antibody in human-derived samples; reactivity with other species is not guaranteed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For LMO2 research in hematopoiesis and leukemia, we can also assist in sourcing rare-target raw materials and custom development needs. Let CamelBio streamline your transition from concept to clinic with comprehensive supply and consulting support.