Anti-LMO1 (Rhombotin-1) Rabbit Polyclonal Antibody for WB, ELISA - P25800

Core Product Specifications and Parameters

Biological Background: Rhombotin-1 (LMO1) Function and Localization

• Rhombotin-1 (LMO1) is a cysteine-rich LIM domain-containing protein, also known as TTG-1, RBTN1, or RHOM1, encoded by the LMO1 gene in humans.
• It may function as a transcriptional regulator in neural lineage cells, potentially through direct DNA binding or interaction with other transcription factors.
• LMO1 belongs to the LIM-only protein family, characterized by LIM domains that mediate protein-protein interactions and coordinate zinc ions.
• The protein is predominantly localized to the nucleus, consistent with its role in gene regulation.
• Expression is highest in the central nervous system, with low levels detected in other tissues such as the thymus.
• Alternative splicing generates multiple isoforms, and LMO1 is classified as a proto-oncogene due to its involvement in chromosomal rearrangements in T-cell acute lymphoblastic leukemia.
• The two tandem LIM domains are essential for its function, serving as modular protein-binding interfaces.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human LMO1 (amino acids 1-156), suggesting the antibody may detect all isoforms. Validate specificity in relevant cell or tissue lysates, particularly neural or thymic samples.
• For WB, a band at ~18 kDa is expected under reducing conditions. Optimize loading and antibody dilution based on target abundance.
• In ELISA, consider using recombinant LMO1 protein as a standard and titrate the antibody for optimal signal-to-noise ratio in your assay system.
• Cross-reactivity with other species has not been tested; confirm in non-human samples if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our one-stop sourcing platform supports diagnostic manufacturers and researchers working on transcription factors and leukemia biomarkers, such as LMO1. Partner with CamelBio to streamline your supply chain from concept to clinical application.