Anti-LMBRD1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NUN5

Core Product Specifications and Parameters

Biological Background: LMBRD1 Function and Localization

• Lysosomal cobalamin transport escort protein LMBD1, also known as NESI, LMBR1 domain-containing protein 1, and nuclear export signal-interacting protein; encoded by the LMBRD1 gene (C6orf209).
• Lysosomal membrane chaperone required for the export of cobalamin (vitamin B12) from the lysosome to the cytosol, enabling its conversion to cofactors (PubMed:19136951). It targets the ABCD4 transporter from the endoplasmic reticulum to the lysosome (PubMed:27456980) and forms a complex with ABCD4 and cytoplasmic MMACHC to transport cobalamin across the lysosomal membrane (PubMed:25535791). Related references: PMID:19136951 PMID:27456980 PMID:25535791
• Acts as an adapter protein playing an important role in mediating and regulating the internalization of the insulin receptor (INSR) via clathrin-mediated endocytosis through its interaction with adapter protein complex 2 (By similarity).
• Essential for the initiation of gastrulation and early formation of mesoderm structures during embryogenesis (By similarity).
• May play a role in the assembly of hepatitis delta virus (HDV) (Microbial infection).
• Subcellular localization includes endoplasmic reticulum membrane, lysosome membrane, cell membrane, and clathrin-coated vesicles.
• Isoform 3 is expressed in liver.
• Additional features: alternative splicing, glycoprotein, phosphoprotein, transmembrane protein with nine predicted transmembrane helices.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 220-300, a region within the protein; consider this when designing detection or competition experiments.
• For Western blotting, a band near 61 kDa (predicted) is expected; validate with appropriate positive control lysates from human or mouse cells.
• In immunofluorescence, co-staining with lysosomal markers can help confirm proper localization.
• As a polyclonal antibody, lot-to-lot variability should be monitored; consider pre-adsorption or affinity purification if high specificity is required.
• The antibody is listed for ELISA; optimize coating and detection conditions for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. For targets such as LMBRD1, involved in lysosomal vitamin B12 transport and insulin receptor trafficking, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Our sourcing capabilities extend to rare targets, custom antibody generation, and full technical consultation from concept to clinic.