Anti-LMAN2L Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H0V9

Core Product Specifications and Parameters

Biological Background: LMAN2L Function and Localization

• LMAN2L (VIP36-like protein), also known as lectin mannose-binding 2-like, is encoded by the LMAN2L gene and belongs to the L-type lectin family, sharing structural features with ERGIC-53 and VIP36.
• The protein contains a signal peptide, a lectin domain with a metal-binding site, a transmembrane helix, and a short cytoplasmic tail, and it undergoes alternative splicing to produce isoforms.
• LMAN2L is a glycoprotein that forms disulfide bonds and localizes predominantly to the endoplasmic reticulum membrane and the Golgi apparatus membrane.
• Its primary function involves the regulation of glycoprotein export from the endoplasmic reticulum, potentially acting as a regulator of ERGIC-53-mediated trafficking.
• Tissue expression is widespread, with the highest levels observed in skeletal muscle and kidney, intermediate levels in heart, liver, and placenta, and lower levels in brain, thymus, spleen, small intestine, and lung.
• Keywords associated with the protein include Endoplasmic reticulum, Golgi apparatus, Lectin, Glycoprotein, Membrane, Metal-binding, Disulfide bond, and Alternative splicing; disease variants are linked to intellectual disability (MRD69, MRT52).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 189-348 of human LMAN2L, a region that may be conserved; verify reactivity in the desired species model (human, mouse, rat).
• For western blotting, a band near 40 kDa is expected (predicted molecular weight ~39.7 kDa); use appropriate protein ladders and loading controls.
• In immunofluorescence/ICC, LMAN2L should display a perinuclear, reticular pattern consistent with ER and Golgi staining; include permeabilization and appropriate organelle markers to confirm localization.
• When developing ELISA assays, consider the antibody's polyclonal nature and validate sensitivity and specificity with recombinant LMAN2L or cell lysates.
• Always include negative controls (e.g., knockdown or knockout samples) to confirm specificity, as polyclonal antibodies may exhibit batch-to-batch variation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated B2B partner for IVD raw materials, supporting diagnostic manufacturers from concept to clinic. Beyond this LMAN2L polyclonal antibody for glycoprotein trafficking research, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets. Let CamelBio streamline your supply chain so you can focus on accelerating reliable diagnostic development.