Anti-LMAN2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q12907

Core Product Specifications and Parameters

Biological Background: LMAN2 Function and Localization

• LMAN2 (Lectin Mannose-Binding 2) encodes the vesicular integral-membrane protein VIP36, also known as GP36b, a member of the legume lectin family that functions as an intracellular carbohydrate-binding protein.
• It acts as a lectin in the early secretory pathway, interacting with high-mannose type N-glycans and potentially O-linked glycans on newly synthesized glycoproteins, thereby facilitating their sorting and transport.
• Subcellularly, LMAN2 localizes primarily to the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and cis-Golgi membranes, with some cycling to the ER, consistent with a role in anterograde cargo selection.
• The protein contains an N-terminal lectin domain that requires calcium for sugar binding, a stalk region, a single-pass transmembrane helix, and a short cytosolic tail that may interact with coat proteins.
• It is ubiquitously expressed across human tissues, underscoring its fundamental importance in constitutive glycoprotein trafficking.
• LMAN2 is a glycoprotein with intrachain disulfide bonds and exists as a homodimer, features important for its stability and function.

Experimental Guidance and Technical Tips

• The immunogen (aa 190–320) maps to the membrane-proximal region of the luminal domain; this segment is highly conserved between human and mouse (95% identity), which supports the observed cross-reactivity with mouse tissues.
• For Western blotting, a protein band of approximately 40 kDa is expected; endoglycosidase treatment (e.g., PNGase F) may be used to confirm glycosylation status and reduce heterogeneity.
• In immunofluorescence/ICC, permeabilization with 0.1% Triton X-100 or digitonin is required; a perinuclear reticular and punctate staining pattern corresponding to ER/Golgi compartments should be visualized.
• This polyclonal antibody can be applied in ELISA as a capture or detection reagent; optimal working concentrations must be determined experimentally for each assay system.
• Pre-adsorption with the immunogen peptide or validation in LMAN2 knockout lysates is recommended to verify specificity in critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and accessories, including validated antibodies, bulk ancillary reagents, and customized sourcing for even the most challenging targets. This anti-LMAN2 polyclonal antibody exemplifies our commitment to supporting glycoprotein trafficking and cell biology research with high-quality reagents. Whether you need ready-to-use antibody pairs, monoclonal alternatives, or rare target sourcing, our one-stop platform streamlines your path from concept to clinic.