Anti-LMAN1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P49257

Core Product Specifications and Parameters

Biological Background: LMAN1 Function and Localization

• Encoded by the LMAN1 gene, Protein ERGIC-53 (also known as gp58, MR60, or lectin mannose-binding 1) is a 510-amino-acid transmembrane protein with a predicted molecular weight of ~57.5 kDa and an observed band at approximately 54 kDa.
• Functions as a mannose-specific lectin that likely recognizes sugar residues on glycoproteins, glycolipids, or GPI anchors, and participates in protein sorting and recycling within the early secretory pathway.
• Forms a specific ER-to-Golgi cargo receptor complex with MCFD2, mediating the forward transport of selected glycoproteins (e.g., coagulation factors V and VIII) from the endoplasmic reticulum to the Golgi.
• Ubiquitously expressed across human tissues, consistent with its fundamental role in intracellular trafficking.
• Subcellular localization: cycles between the ER-Golgi intermediate compartment (ERGIC), Golgi apparatus, and endoplasmic reticulum membranes.
• Post-translational modifications include phosphorylation and disulfide bond formation; contains a single transmembrane helix and requires metal binding for proper lectin function.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant fragment spanning amino acids 270–480 of human LMAN1 (NP_005561.1), which corresponds to a large portion of the protein’s luminal domain; this region may present conformational epitopes favorable for detecting endogenous LMAN1.
• For Western blot (WB), a band around 54 kDa is expected in lysates from human, mouse, or rat cells; optimize loading and antibody dilution using positive controls (e.g., HeLa or liver tissue) known to express ERGIC-53.
• In immunohistochemistry (IHC-P), consider antigen retrieval with citrate buffer (pH 6.0) and test on formalin-fixed paraffin-embedded sections of tissues like liver, pancreas, or colon where LMAN1 is abundant.
• For immunofluorescence (IF/ICC), use a permeabilization step to detect the protein in ERGIC/ER/Golgi membranes; co-staining with organelle markers (e.g., GM130 for cis-Golgi) can confirm the characteristic perinuclear reticular pattern.
• In ELISA applications, the polyclonal nature of this antibody may provide broad epitope coverage, but dilution and blocking conditions must be empirically optimized for the specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supplying validated antibodies like this LMAN1 polyclonal antibody to support intracellular trafficking research and diagnostic assay development. Beyond this product, we offer optimized antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents, plus customized sourcing for challenging targets to help bridge your workflow from concept to clinic.