Anti-LLPH Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BRT6

Core Product Specifications and Parameters

Biological Background: LLPH Function and Localization

• LLPH (Protein LLP homolog, also known as LAPS18-like) is a 15 kDa nuclear protein encoded by the C12orf31 gene.
• Functionally, LLPH promotes dendritic and spine growth and regulates synaptic transmission in hippocampal neurons.
• It localizes predominantly to the nucleolus and chromosomes, suggesting roles in chromatin-related processes.
• The protein contains features indicative of potential ubiquitin-like modifications (isopeptide bond, Ubl conjugation).
• Structural studies (3D-structure keyword) indicate it has a defined fold, possibly contributing to interactions with nucleic acids or proteins.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human LLPH (amino acids 1-129), so the antibody is expected to detect the complete protein.
• For Western blotting, the predicted molecular weight is 15 kDa; use a 15% SDS-PAGE gel and transfer to a low-fluorescence PVDF membrane.
• In immunofluorescence/ICC, anticipate nuclear and nucleolar localization; confirm staining pattern with a nuclear marker.
• As a polyclonal antibody, verify lot-to-lot consistency by testing on a known positive control sample.
• Consider using human SH-SY5Y neuroblastoma cells or rat hippocampal neuron cultures as positive controls.
• For ELISA, use the recombinant immunogen or a peptide standard for quantitative calibration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated antibodies, antigen pairs, and ancillary reagents under one roof. Our portfolio supports neuroscientific targets like LLPH, with customizable polyclonal antibodies and bulk reagent supply. Partner with us to bridge your concept-to-clinic development with dedicated technical and sourcing support.