Anti-LLGL1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q15334

Core Product Specifications and Parameters

Biological Background: LLGL1 Function and Localization

• LLGL1 (Lethal(2) giant larvae protein homolog 1) is a homolog of the Drosophila tumor suppressor gene l(2)gl and is also known as Hugl-1, DLG4, and HUGL1.
• It functions as a cortical cytoskeleton protein that maintains cell polarity and epithelial integrity by participating in a protein complex at the plasma membrane.
• LLGL1 regulates mitotic spindle orientation, proliferation, differentiation, and tissue organization of neuroepithelial cells, playing a critical role in neurodevelopment.
• It is involved in axonogenesis by activating RAB10, thereby regulating vesicular membrane trafficking toward the axonal plasma membrane.
• The protein localizes to early endosome membranes, Golgi apparatus (including trans-Golgi network), cell projections/axons, and associates with the cytoskeleton.
• Tissue expression is highest in brain, kidney, and muscle, while it is barely detected in heart and placenta; importantly, LLGL1 is downregulated or lost in many tumor cell lines and advanced-stage tumors.
• LLGL1 contains WD repeats and is subject to phosphorylation, with a molecular weight of approximately 115 kDa.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal region (amino acids 1–190) of human LLGL1; consider verifying specificity by comparing staining patterns in LLGL1-positive vs. knockdown/knockout models.
• For Western blot, the predicted molecular weight of LLGL1 is ~115 kDa; include appropriate molecular weight markers and validate detection in lysates from brain or kidney tissues.
• IHC-P may require optimization of antigen retrieval conditions (e.g., pH6 citrate buffer vs. pH9 EDTA) depending on tissue type and fixation.
• When using IF/ICC, note that LLGL1 associates with membranes and cytoskeletal structures, so permeabilization protocols should be carefully optimized to preserve epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to IVD raw materials, technical services, and consulting. This anti-LLGL1 antibody supports studies of cell polarity and tumor suppression, areas relevant to neurobiology and oncology. As a one-stop sourcing partner, CamelBio offers validated antibody pairs, bulk ancillary reagents, and customized procurement for challenging targets, helping you streamline development from concept to clinic.