Anti-LKB1 Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q15831

Core Product Specifications and Parameters

Biological Background: LKB1 Function and Localization

• Serine/threonine-protein kinase STK11 (LKB1) acts as a tumor suppressor by controlling AMPK family activity, regulating cell metabolism, polarity, apoptosis, and DNA damage response. Related references: PMID:12805220, PMID:14517248, PMID:14976552

• Functions as a key upstream regulator of AMPK, phosphorylating PRKAA1/PRKAA2 to inhibit cell growth, maintain glucose homeostasis, activate autophagy, and mediate DNA damage-dependent B-cell differentiation. Related references: PMID:14976552

• Phosphorylates AMPK-related kinases BRSK1/2 to drive cortical neuron polarization and axon specification, and remodels the actin cytoskeleton to establish cellular polarity. Related references: PMID:14976552, PMID:15016379

• Participates in DNA damage response by interacting with p53, recruiting to the CDKN1A promoter, and regulating UV-induced DNA repair through CDKN1A phosphorylation with NUAK1. Related references: PMID:11430832, PMID:17108107, PMID:21317932, PMID:25329316

• Plays a role in spermiogenesis.

• Localizes to the nucleus, cytoplasm, membrane, mitochondrion, and late endosome.

• Ubiquitously expressed, with highest levels in testis and fetal liver.

• Post-translational modifications include acetylation, palmitoylation, prenylation, phosphorylation, and methylation, indicating complex regulatory mechanisms.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 134–433 of human LKB1; consider its suitability for detecting full-length and truncated isoforms.
• For western blotting (WB), an expected band near 49 kDa may be observed; actual migration can vary due to modifications.
• For immunocytochemistry (IF/ICC) and immunohistochemistry (IHC-P), optimize fixation, permeabilization, and antigen retrieval conditions for your sample.
• The antibody cross-reacts with mouse and rat, enabling cross-species studies; validate in the relevant model system.
• As a polyclonal antibody, performance may vary between lots; we recommend routine validation with positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by supplying validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing for diagnostic developers. This anti-LKB1 polyclonal antibody supports research into tumor biology, metabolic signaling, and cellular polarity, and comes with flexible supply options to match your assay development pipeline. Contact us to discuss how our end-to-end raw-material services can accelerate your project from concept to clinic.