Anti-Lipoprotein lipase (LPL) Rabbit Polyclonal Antibody for WB, ELISA - P06858

Core Product Specifications and Parameters

Biological Background: Lipoprotein lipase Function and Localization

• Key enzyme in triglyceride metabolism: catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), thereby playing a critical role in lipid clearance, utilization, and storage. Related references: PMID:11342582, PMID:27578112, PMID:8675619
• Although it possesses both phospholipase and triglyceride lipase activities, LPL primarily functions as a triglyceride lipase with only low but detectable phospholipase activity. Related references: PMID:12032167, PMID:7592706
• Mediates margination of triglyceride-rich lipoprotein particles in capillaries. Related references: PMID:24726386
• Recruited to the luminal surface of vascular endothelium via binding to GPIHBP1 and cell surface heparan sulfate proteoglycans. Related references: PMID:11342582, PMID:27811232
• Subcellular localization includes cell membrane, secreted extracellular space, and extracellular matrix.
• Detected in blood plasma and milk at the protein level. Related references: PMID:11893776, PMID:12641539, PMID:2340307
• Belongs to the AB hydrolase superfamily (lipase family). Contains a signal peptide, multiple disulfide bonds, and is a glycoprotein with heparin-binding and calcium/metal-binding properties.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 124–475, a conserved region that likely contributes to cross-reactivity with mouse and rat LPL.
• For western blotting, the predicted molecular weight is ~53 kDa; consider using appropriate positive controls and reducing conditions for optimal detection.
• In ELISA and other immunoassays, validate the antibody with recombinant LPL or native samples in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials. For lipid metabolism and cardiovascular biomarker development, we offer polyclonal antibodies like this anti-LPL product, along with validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and sourcing support for rare targets—helping you advance from concept to clinic efficiently.