Anti-Lipoprotein lipase (LPL) Rabbit Monoclonal Antibody for WB, ELISA - P06858

Core Product Specifications and Parameters

Biological Background: Lipoprotein lipase Function and Localization

• Lipoprotein lipase (LPL) is a key enzyme in triglyceride metabolism, catalyzing the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), thereby playing a critical role in lipid clearance, utilization, and storage. Related references: PMID:11342582, PMID:27578112, PMID:8675619
• Although LPL possesses both phospholipase and triglyceride lipase activities, it functions primarily as a triglyceride lipase with detectable phospholipase activity. Related references: PMID:12032167, PMID:7592706
• LPL mediates margination of triglyceride-rich lipoprotein particles in capillaries, facilitating efficient lipolysis. Related references: PMID:24726386
• The enzyme is recruited to the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans. Related references: PMID:11342582, PMID:27811232
• Subcellular localization includes the cell membrane, secretion into the extracellular space, and association with the extracellular matrix.
• LPL is detected in blood plasma and milk, indicating its presence in both systemic circulation and secretory fluids.
• The protein contains disulfide bonds, is glycosylated, and requires calcium and heparin binding for its function; it is associated with disease variants linked to hyperlipidemia.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 376–475 of human LPL (C-terminal region). When using for western blot, consider running samples under reducing conditions and anticipate a band at approximately 53 kDa.
• Cross-reactivity with mouse suggests this antibody can be used in mouse tissue or cell lysates; confirm specificity in the relevant species.
• For ELISA applications, optimize antibody concentration and validate using recombinant LPL protein or known positive control samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable partner for IVD raw material sourcing, offering a comprehensive portfolio of validated antibodies, antigens, and ancillary reagents to accelerate diagnostic assay development. This anti-LPL monoclonal antibody supports research in lipid metabolism and cardiovascular disease biomarker detection. Whether you need customized antibody pairs, bulk volumes, or rare target sourcing, our team is ready to bridge your procurement needs from concept to clinic.