Anti-Lipocalin-2/NGAL Rabbit PolymAb® for WB, IF-P, IHC-P, ELISA - P80188 / P11672

Core Product Specifications and Parameters

Biological Background: Lipocalin-2/NGAL Function and Localization

• Lipocalin-2/NGAL (LCN2) is a 25 kDa secreted glycoprotein also known as siderocalin, p25, and oncogene 24p3.
• Functions as an iron-trafficking protein: binds iron via siderophores such as 2,3-DHBA and mediates iron delivery (holo-form) or removal (apo-form), influencing intracellular iron concentration. Related references: PMID:12453413, PMID:20581821, PMID:27780864
• Iron-free apo-LCN2 induces apoptosis by decreasing intracellular iron and upregulating proapoptotic BCL2L11/BIM.
• Key innate immunity component: sequesters bacterial siderophores (e.g., enterobactin) to limit iron availability for pathogens; also binds Mycobacterium tuberculosis siderophores. Related references: PMID:27780864, PMID:15642259, PMID:21978368
• Subcellular localization includes secreted, cytoplasmic granule lumen, and cytoplasmic vesicle lumen.
• Strongly expressed in neutrophils, bone marrow, and epithelial tissues including uterus, prostate, salivary gland, stomach, appendix, colon, trachea, lung, and kidney medullary tubules. Related references: PMID:7683678, PMID:8298140, PMID:9339356, PMID:30418175
• Post-translational modifications include glycosylation, disulfide bonds, and pyrrolidone carboxylic acid formation.

Experimental Guidance and Technical Tips

• The antibody is raised against recombinant human/mouse Lipocalin-2/NGAL, making it suitable for detecting both endogenous and overexpressed forms.
• For western blot (WB), a band at approximately 23 kDa is expected; consider using reducing conditions.
• For immunohistochemistry (IHC-P), antigen retrieval may be required; validate in your tissue system.
• For immunofluorescence (IF-P), optimize permeabilization and fixation protocols due to secreted and vesicular localization.
• As a polymorphic monoclonal antibody (PolymAb®), it offers consistency; still, validate in your specific assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting from concept to clinic. This anti-Lipocalin-2/NGAL antibody supports research in apoptosis, innate immunity, iron metabolism, and renal biology. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare-target raw materials to accelerate your diagnostic development.