Anti-Lipin 1 Rabbit Polyclonal Antibody for WB, ELISA - Q14693

Core Product Specifications and Parameters

Biological Background: Lipin 1 Function and Localization

• Lipin 1 (LPIN1) is a magnesium-dependent phosphatidate phosphatase that catalyzes the conversion of phosphatidic acid to diacylglycerol, a key step in triglyceride and phospholipid biosynthesis. Related references: PMID:20231281, PMID:23426360, PMID:29765047
• It plays a critical role in adipocyte differentiation and lipid metabolism, acting as a transcriptional coactivator for PPARGC1A/PPARA to regulate lipid metabolic gene expression.
• LPIN1 is recruited to the mitochondrial outer membrane and promotes mitochondrial fission through its enzymatic activity.
• The protein localizes to the cytoplasm, endoplasmic reticulum membrane, and nucleus membrane, consistent with its dual roles in lipid synthesis and gene regulation.
• Tissue expression is highest in skeletal muscle and adipose tissue, with lower levels in parts of the digestive tract, reflecting its metabolic functions.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination; alternative splicing generates multiple isoforms.
• Key associated keywords: Fatty acid metabolism, Lipid metabolism, Transcription regulation, Metal-binding, Nucleus, Endoplasmic reticulum.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 261-414) lies within a conserved central domain of Lipin 1, which aligns with the reported cross-reactivity to mouse and rat. Consider this when designing species-specific experiments.
• For Western blot, a band near the expected molecular weight of ~99 kDa should be observed; verify specificity using appropriate positive and negative controls.
• For ELISA applications, the antibody may be used as a capture or detection reagent; validate optimal working concentrations and pairing in your assay system.
• Due to the protein's multiple subcellular localizations, sample preparation and fractionation protocols may influence detection; consider including organelle-specific markers if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a streamlined one-stop sourcing solution for IVD raw materials, covering every stage from concept to clinic. This Lipin 1 polyclonal antibody supports research in lipid metabolism, adipocyte biology, and metabolic disorders. Beyond single reagents, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your assay development workflows.