Anti-Lipin 1 Rabbit Polyclonal Antibody for WB and ELISA - Q14693

Core Product Specifications and Parameters

Biological Background: Lipin 1 Function and Localization

• LPIN1 functions as a magnesium-dependent phosphatidate phosphatase, converting phosphatidic acid to diacylglycerol, a key step in triglyceride, phosphatidylcholine, and phosphatidylethanolamine biosynthesis. Related references: PMID:20231281, PMID:23426360, PMID:29765047
• LPIN1 is involved in adipocyte differentiation, acting downstream of key adipogenic transcription factors.
• It localizes to the outer mitochondrial membrane, promoting mitochondrial fission through phosphatidic acid conversion.
• In the nucleus, LPIN1 serves as a transcriptional coactivator for PPARGC1A/PPARA, modulating lipid metabolism gene expression.
• LPIN1 is predominantly expressed in skeletal muscle and adipose tissue, with lower levels in the digestive tract.
• The protein undergoes multiple post-translational modifications including acetylation and phosphorylation, and exists in several alternatively spliced isoforms.
• It is an important regulator of fatty acid metabolism and is listed under keywords such as Hydrolase, Lipid metabolism, and Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 261-414 of human LPIN1; consider this internal region for epitope recognition when designing detection strategies.
• For Western blot, optimize sample preparation and antibody dilution, taking into account the predicted molecular weight of approximately 99 kDa.
• Given cross-reactivity with mouse and rat, this antibody can be utilized for comparative studies across these species; validate in the relevant sample system beforehand.
• ELISA suitability is indicated; confirm optimal coating and detection conditions experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a streamlined channel to IVD raw materials, including validated polyclonal antibodies for lipid metabolism targets such as LPIN1. Our comprehensive supply portfolio covers monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare target raw materials. We help accelerate your development from concept to clinical application with reliable, one-stop procurement support.