Anti-LIPH Rabbit Polyclonal Antibody for WB & ELISA - Q8WWY8

Core Product Specifications and Parameters

Biological Background: LIPH Function and Localization

• LIPH (Lipase member H) is a secreted and membrane-associated phospholipase A1, also referred to as mPA-PLA1 alpha or PLA1B.
• It selectively hydrolyzes phosphatidic acid (PA) to generate 2-acyl lysophosphatidic acid (LPA) and free fatty acid; it does not act on other phospholipids or triglycerides.
• At the protein level, it is present in the intestine, and its mRNA is broadly expressed in the colon, prostate, kidney, pancreas, ovary, testis, lung, and skin. In the skin, it is prominently expressed in hair follicles, including the stem cell-rich bulge region and inner root sheath. Related references: PMID:17095700, PMID:32336749
• The protein localizes to the cell membrane and is also secreted into the extracellular space.
• Mutations in the LIPH gene are associated with hypotrichosis, highlighting its importance in hair follicle biology.
• LIPH is a glycoprotein with disulfide bonds and heparin-binding affinity, features that contribute to its structural stability and interaction properties.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to amino acids 350–450 of human LIPH; cross-reactivity with mouse and rat suggests epitope conservation across species.
• In Western blotting, a band near 51 kDa is expected; however, glycosylation or other modifications may shift the apparent molecular weight. A standard reducing SDS-PAGE protocol is recommended.
• For ELISA applications, consider using recombinant LIPH protein or tissue lysates known to express LIPH (e.g., intestine, skin) for plate coating or as standards.
• Positive control samples could include lysates from LIPH-rich tissues such as intestine or hair follicle cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. This anti-LIPH polyclonal antibody complements our portfolio of lipid metabolism and dermatological targets, aiding assay development for LPA-mediated signaling and hair growth studies. Our one-stop sourcing includes validated antibody pairs, bulk reagents, and customized solutions to streamline your procurement process.