Anti-LIPG Polyclonal Antibody for WB, ELISA - Q9Y5X9

Core Product Specifications and Parameters

Biological Background: Endothelial Lipase Function and Localization

• Endothelial lipase (LIPG) is a secreted enzyme that exhibits both phospholipase A1 and triglyceride lipase activities, with higher phospholipase activity (PMID:10192396, PMID:10318835, PMID:12032167).
  Related references: PMID:10192396, PMID:10318835, PMID:12032167
• Hydrolyzes triglycerides with both short-chain (tributyrin) and long-chain (triolein) fatty acyl groups with similar efficiencies (PMID:12032167).
  Related references: PMID:12032167
• Preferentially hydrolyzes high-density lipoproteins (HDL) over other lipoproteins (PMID:10192396, PMID:12032167).
  Related references: PMID:10192396, PMID:12032167
• Subcellular localization: Secreted into the extracellular space.
• Highly expressed in liver, placenta, lung, thyroid, kidney, testis, corpus luteum, and endothelial cells; not detected in heart, brain, or muscle.
• Contains disulfide bonds, glycosylation sites, and a heparin-binding domain; involved in lipid degradation and metabolism.
• Molecular weight of the mature protein is approximately 57 kDa; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 251–500 of human LIPG; the antibody may recognize linear epitopes within this region. Verify specificity in your experimental system using appropriate controls.
• Predicted band size is 57 kDa in Western blotting; slight shifts may occur due to glycosylation or other post-translational modifications.
• Cross-reactivity with mouse LIPG is indicated; validate in mouse-derived samples if used for cross-species applications.
• For ELISA, optimize coating and detection conditions to achieve optimal signal-to-noise ratio.
• As a polyclonal antibody, it may exhibit batch-to-batch variation; consider using the same lot for long-term comparative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. For researchers focused on lipid metabolism and cardiovascular biomarkers, we provide this anti-LIPG polyclonal antibody alongside rare-target sourcing and technical consultation, supporting your workflow from concept to clinic.