Anti-LIPF Rabbit Polyclonal Antibody for WB and ELISA - P07098

Core Product Specifications and Parameters

Biological Background: Gastric triacylglycerol lipase (LIPF) Function and Localization

• The LIPF gene product, also known as gastric lipase (GL), human gastric lipase (HGL), and HLAL, is a secreted enzyme essential for dietary fat breakdown.
• Catalyzes the hydrolysis of triacylglycerols to yield free fatty acids, diacylglycerol, monoacylglycerol, and glycerol, with a preference for the sn-3 position (PubMed:10358049, PubMed:2243091). Related references: PMID:10358049 PMID:2243091
• Secreted into the gastric lumen, where it initiates the digestion of dietary lipids under acidic conditions.
• Contains a signal peptide, disulfide bonds, and N-glycosylation sites; alternative splicing produces multiple isoforms (UniProt keywords: Alternative splicing, Disulfide bond, Glycoprotein, Signal).
• The predicted molecular weight is approximately 45 kDa (UniProt: 45238 Da), matching the observed protein weight of 45 kDa.
• Belongs to the AB hydrolase superfamily and plays a central role in lipid degradation and metabolism (keywords: Hydrolase, Lipid degradation, Lipid metabolism).

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 219-398 of human LIPF, a region within the mature enzyme; consider using full-length recombinant LIPF as a positive control in your assays.
• For western blot (WB) analysis, gastric tissue lysates or recombinant protein can be used; the expected band size is approximately 45 kDa.
• Cross-reactivity with mouse and rat is reported; validate antibody specificity and sensitivity in your individual sample matrix and application.
• Optimize primary antibody dilution, blocking buffer, and incubation conditions based on your experimental system and detection method.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibodies, recombinant proteins, and ancillary reagents for lipid metabolism research. From concept to clinic, we provide optimized monoclonal and polyclonal antibodies like this LIPF rabbit pAb, as well as bulk reagents and custom sourcing for rare targets. Streamline your diagnostic development with our comprehensive supply chain and technical expertise.