Anti-LIPA Polyclonal Antibody for WB, ELISA - P38571

Core Product Specifications and Parameters

Biological Background: Lysosomal Acid Lipase/Cholesteryl Ester Hydrolase (LIPA) Function and Localization

• Lysosomal acid lipase/cholesteryl ester hydrolase (LIPA), also known as LAL or CESD, is encoded by the LIPA gene; alternative splicing produces multiple isoforms.
• Catalyzes the deacylation of cholesteryl ester core lipids from endocytosed low-density lipoproteins within the lysosome, releasing free cholesterol and fatty acids essential for cellular lipid homeostasis. Related references: PMID:15269241, PMID:1718995, PMID:7204383
• Hydrolyzes triglycerides and diglycerides with preference for acyl moieties at the sn-1 or sn-3 positions, contributing to intracellular lipid degradation. Related references: PMID:7204383, PMID:8112342
• Predominantly localized to lysosomes, consistent with its role in processing endocytosed lipoproteins.
• Highly expressed in brain, lung, kidney, and mammary gland; moderate in placenta; low in liver and heart.
• Classified as a glycoprotein and a member of the AB hydrolase superfamily; includes a signal peptide and is subject to proteolytic processing.
• Mutations in LIPA cause lysosomal acid lipase deficiency, which manifests as Wolman disease or cholesteryl ester storage disease, underscoring its clinical relevance in lipid metabolism disorders.

Experimental Guidance and Technical Tips

• The immunogen spans the central region (aa 110-399) of human LIPA, encompassing the catalytic domain; this region is highly conserved between human and rat, supporting detection in both species.
• For Western blotting, expect a specific band near 45 kDa; validation in lysates from brain, lung, or kidney tissues is recommended due to high LIPA expression.
• In ELISA assays, consider pairing with a capture antibody to improve sensitivity; optimal antibody concentrations should be determined by titration in the relevant sample matrix.
• The antibody has not been tested in immunohistochemistry; if required, preliminary testing with appropriate antigen retrieval methods is advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, providing diagnostic manufacturers, laboratories, and research institutes with streamlined access to validated antibodies and ancillary reagents. This anti-LIPA polyclonal antibody supports investigations into lipid metabolism and lysosomal storage disorders; to accelerate your assay development, we also offer optimized monoclonal/polyclonal pairs, bulk recombinant proteins, and sourcing solutions for rare targets. Let CamelBio bridge your concept-to-clinic journey with reliable material supply and technical guidance.