Anti-LINS1 Rabbit Polyclonal Antibody for WB, ELISA - Q8NG48

Core Product Specifications and Parameters

Biological Background: LINS1 Function and Localization

• LINS1, also known as Lines homolog 1 or WINS1, is a protein encoded by the LINS1 gene in humans, with a predicted molecular weight of approximately 86 kDa.
• It is expressed in adult testis, prostate, spleen, thymus, skeletal muscle, fetal kidney and brain, indicating a potential role in both developmental and adult tissue functions.
• The protein is annotated as a Wnt-signaling molecule, suggesting involvement in the Wnt pathway, which is critical for embryonic development, cell proliferation, and differentiation.
• Variants in LINS1 are associated with autosomal recessive intellectual disability (MRT27), highlighting its importance in cognitive development.
• Post-translational modifications include phosphorylation, as indicated by its classification as a phosphoprotein, which may regulate its activity or interactions.
• The gene undergoes alternative splicing, potentially generating multiple isoforms with distinct expression patterns or functions.

Experimental Guidance and Technical Tips

• The immunogen was designed from the C-terminal region (amino acids 541–700) of human LINS1; therefore, the antibody is expected to recognize full-length LINS1 and possibly other isoforms containing this region.
• For Western blot, a band around 86 kDa is predicted; however, due to alternative splicing or post-translational modifications, additional bands may be observed in certain sample types.
• Given the cross-reactivity with mouse and rat, this antibody can be used for comparative studies across these species, but validation in your specific model system is recommended.
• For ELISA, consider using the recombinant protein as a positive control to assess antibody binding and titer.
• As a polyclonal antibody, lot-to-lot variability may exist; it is advisable to optimize dilution and incubation conditions for each new lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials. For LINS1-related studies, we offer this validated rabbit polyclonal antibody along with complementary reagents such as blocking buffers and secondary antibodies. Our sourcing expertise extends to rare targets, enabling seamless procurement of all necessary components for your research or diagnostic development.