Anti-LIN9 Polyclonal Antibody for WB, ELISA - Q5TKA1

Core Product Specifications and Parameters

Biological Background: LIN9 Function and Localization

• LIN9 (Protein lin-9 homolog) is a tumor suppressor that inhibits DNA synthesis and oncogenic transformation through interaction with the retinoblastoma protein RB1.
• It plays a critical role in regulating the G1/S transition by controlling the expression of cell cycle genes.
• Also known as Beta subunit-associated regulator of apoptosis (BARA), TUDOR gene similar protein 1/2 (TGS1/TGS2), and pRB-associated protein, reflecting its diverse cellular associations.
• The LIN9 protein is localized exclusively in the nucleoplasm, consistent with its role in transcriptional regulation and cell cycle control.
• LIN9 belongs to a gene family that includes alternative splicing variants, and it features a coiled-coil domain important for protein-protein interactions.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin conjugation (isopeptide bond), which likely modulate its stability and activity.
• Although broadly relevant to cell cycle research, LIN9 expression is notably high in thymus and testis, suggesting specific roles in these tissues.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 350–470 of human LIN9, a region outside known coiled-coil domains; consider verifying epitope accessibility in your specific assay format.
• For Western blotting, the expected band size is approximately 62 kDa based on UniProt data; validate molecular weight using appropriate human or mouse cell lysates as positive controls.
• In ELISA applications, using recombinant LIN9 protein fragment as a coating antigen may help optimize signal-to-noise ratios; titrate antibody for best results.
• Since cross-reactivity with mouse is indicated, this antibody may be suitable for studies in murine models; confirm performance in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering a comprehensive portfolio that includes validated antibody pairs, optimized polyclonal antibodies like this anti-LIN9 antibody, and bulk ancillary reagents. Our platform simplifies procurement for cell cycle and tumor suppressor research by ensuring consistent supply of hard-to-find target antibodies and related components. From early-stage development to commercial scale-up, CamelBio supports your diagnostic assay needs with reliable materials and technical guidance.