Anti-LIN7C Rabbit Polyclonal Antibody for WB and ELISA - Q9NUP9

Core Product Specifications and Parameters

Biological Background: LIN7C Function and Localization

• LIN7C (Protein lin-7 homolog C, also known as MALS-3, Veli-3) is a member of the LIN7 family that forms membrane-associated multiprotein complexes at polarized cell junctions.
• It plays a critical role in establishing and maintaining asymmetric distribution of channels and receptors, such as NMDA receptor subunit NR2B (GRIN2B) and Kir2 channels, at the plasma membrane.
• The tripartite complex of LIN7 (LIN7A/B/C), CASK, and APBA1 associates with the motor protein KIF17 to transport vesicles containing NMDA receptor NR2B along microtubules, potentially coupling synaptic vesicle exocytosis to cell adhesion.
• LIN7C is essential for proper localization of GRIN2B to neuronal postsynaptic density and is recruited by beta-catenin and cadherin, contributing to synapse organization.
• In epithelial cells, LIN7C is required for basolateral membrane targeting of EGFR/ERBB1, ERBB2, ERBB3, ERBB4 receptors, and the GABA transporter SLC6A12, ensuring polarized protein trafficking.
• Subcellularly, LIN7C localizes to the cell membrane, basolateral cell membrane, cell junctions including tight junctions, and postsynaptic density membrane.
• Key post-translational modifications include acetylation; the protein is associated with processes such as exocytosis, protein transport, and synapse function.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-70) of human LIN7C, which may be conserved across species; cross-reactivity with human, mouse, and rat has been reported.
• For Western blotting (WB), the expected band size is approximately 22 kDa. Consider using a high-percentage gel and appropriate transfer conditions for low-molecular-weight proteins.
• For ELISA, optimize capture or detection conditions with recombinant or native LIN7C, and validate linearity and sensitivity in the relevant matrix.
• As a polyclonal antibody, batch-to-batch variability may occur; always validate specificity in the sample system of interest, using knockdown or knockout controls if available.
• Due to sequence similarity among LIN7 family members (LIN7A, LIN7B), the antibody may exhibit cross-reactivity; if isoform-specific detection is required, confirm with appropriate controls.
• For applications beyond WB and ELISA (e.g., immunofluorescence, IHC), note that LIN7C localizes to cell junctions and membranes; permeabilization may be needed, and fixation conditions should be optimized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to a comprehensive range of IVD raw materials, from validated antibody pairs to ancillary reagents. For investigations into cell polarity, membrane trafficking, and synaptic organization, our LIN7C polyclonal antibody serves as a robust tool for WB and ELISA applications. We also assist in sourcing rare-target antibodies and bulk raw materials, supporting your assay development pipeline from concept to clinic.