Anti-LIN28B Rabbit Monoclonal Antibody for WB, IP, IHC-P, ELISA - Q6ZN17

Core Product Specifications and Parameters

Biological Background: LIN28B Function and Localization

• LIN28B acts as a suppressor of microRNA biogenesis, specifically targeting let-7 family miRNAs. It binds primary let-7 transcripts (pri-let-7) and sequesters them in the nucleolus, shielding them from the microprocessor complex and preventing their maturation (PubMed:22118463).
  Related references:
  PMID:22118463
• This repression of let-7 expression is critical for normal development and helps maintain pluripotency in embryonic stem cells by inhibiting differentiation signals.
  Related references:
  PMID:22118463
• Upon overexpression, LIN28B can recruit ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to terminal uridylation and degradation of the miRNA precursors (PubMed:19703396).
  Related references:
  PMID:19703396
• The protein contains two zinc-finger domains and a cold-shock domain, classifying it as an RNA-binding protein involved in RNA-mediated gene silencing.
• LIN28B is predominantly localized in the nucleolus and nucleus, but can also shuttle to the cytoplasm, which is essential for its function in miRNA processing.
• Tissue expression analysis reveals high levels in the placenta, with lower expression in testis and fetal liver. Distinct isoform expression patterns are observed in hepatocellular carcinoma.
• The gene undergoes alternative splicing, producing at least two isoforms—isoform 1 promotes cell growth while isoform 2 does not, suggesting functional divergence.
• Post-translational modifications include phosphorylation, and the protein is identified as a proteomics marker; it also features a 3D-structure entry.

Experimental Guidance and Technical Tips

• The immunogen for this antibody covers amino acids 1-163 of human LIN28B, targeting the N-terminal region. This region includes the cold-shock domain and is conserved across reported isoforms, potentially enabling detection of both isoform 1 and isoform 2. However, validation in the specific sample system is recommended.
• For Western blot (WB), a band of approximately 27 kDa is expected. Ensure proper protein extraction, as LIN28B is predominantly nuclear and nucleolar; consider using RIPA buffer or nuclear extraction kits to improve solubility.
• In immunohistochemistry (IHC-P), anticipate nuclear and nucleolar staining patterns. Use appropriate antigen retrieval methods and validate staining with positive control tissues such as placenta or cancerous tissues where LIN28B is highly expressed.
• For immunoprecipitation (IP), the rabbit monoclonal format provides high specificity and affinity. Optimize antibody concentration and incubation conditions for the target cell line or tissue lysate.
• This antibody is validated for human samples; cross-reactivity with other species has not been reported. Researchers should verify reactivity if using non-human specimens.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner, providing diagnostic manufacturers, laboratories, and research institutes with essential IVD raw materials throughout the development pipeline—from concept to clinic. For projects involving LIN28B and related cancer or stem cell research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and a full suite of ancillary reagents, ensuring consistent supply and scalability. Whether you need high-performing antibodies for immunoassay development or rare target raw-material sourcing, CamelBio streamlines procurement to accelerate your diagnostic and translational workflows.