Anti-LIN28A Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9H9Z2

Core Product Specifications and Parameters

Biological Background: LIN28A Function and Localization

• LIN28A (Protein lin-28 homolog A, also known as ZCCHC1) is a conserved RNA-binding protein characterized by two cold shock domains and two CCHC zinc finger motifs, placing it in the zinc finger protein family.
• It functions as a suppressor of microRNA biogenesis by specifically binding pre-let-7 miRNAs and other pre-miRNAs at a 5'-GGAG-3' terminal loop motif, thereby recruiting TUT4 and TUT7 uridylyltransferases. Uridylation triggers degradation and prevents processing by Dicer, crucial for maintaining pluripotency (PubMed:18951094, PubMed:19703396, PubMed:22118463). Related references: PMID:18951094 PMID:19703396 PMID:22118463
• LIN28A acts as a translational enhancer by driving specific mRNAs (e.g., IGF2, MYOD1) to polysomes, increasing protein synthesis and mRNA stability, which is essential for skeletal muscle differentiation (PubMed:21247876). Related references: PMID:21247876
• It localizes to multiple subcellular compartments: cytoplasm, rough endoplasmic reticulum, P-bodies, stress granules, and the nucleolus, reflecting its diverse roles in RNA metabolism.
• LIN28A is highly expressed in embryonic stem cells, placenta, and testis, and is frequently up-regulated in HER2-overexpressing breast tumors, linking it to stemness and oncogenesis.
• Post‑translational modifications of LIN28A include acetylation and phosphorylation, which may regulate its activity and protein interactions.
• Additionally, LIN28A binds and enhances the translation of mRNAs encoding metabolic enzymes such as PFKP, PDHA1, and SDHA, thereby increasing glycolysis and oxidative phosphorylation to support tissue repair.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to amino acids 100–209 of human LIN28A. Given the high sequence conservation, cross‑reactivity with mouse LIN28A is expected, consistent with the product’s documented reactivity.
• For Western blot, the predicted molecular weight is ~23 kDa; use appropriate gel percentages (e.g., 12–15% SDS‑PAGE) and include relevant loading controls.
• For immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), validate the antibody in LIN28A‑expressing tissues or cell lines (e.g., embryonic stem cells, testis). Antigen retrieval conditions and antibody dilutions must be optimized empirically.
• Due to its multilocalization, IF/ICC may reveal cytoplasmic and nucleolar staining patterns; co‑staining with organelle markers is recommended to confirm specificity.
• As a polyclonal antibody, lot‑to‑lot consistency should be verified for quantitative assays; consider including positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with efficient, one‑stop access to premium IVD raw materials. This anti‑LIN28A polyclonal antibody supports stem cell and oncology research, reflecting our commitment to delivering high‑quality reagents for critical targets. Beyond antibodies, our extensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—helping you streamline development from concept to clinic.