Anti-LIN28A Monoclonal Antibody for WB, IHC-P, ELISA - Q9H9Z2

Core Product Specifications and Parameters

Biological Background: LIN28A Function and Localization

• LIN28A (Protein lin-28 homolog A, also known as Zinc finger CCHC domain-containing protein 1) is an RNA-binding protein that suppresses let-7 microRNA biogenesis and translationally regulates mRNAs critical for developmental timing, pluripotency, and metabolism (PubMed:21247876). Related references: PMID:21247876
• It specifically binds the terminal loop of pre-let-7 miRNAs via a 5'-GGAG-3' motif, recruiting TUT4/TUT7 uridylyltransferases to add uridine tails, blocking Dicer processing and marking pre-miRNAs for degradation (PubMed:18951094, 19703396, 22118463). Related references: PMID:18951094 PMID:19703396 PMID:22118463
• As a translational enhancer, LIN28A drives specific mRNAs (e.g., IGF2, MYOD1) to polysomes, increasing protein synthesis efficiency, which is essential for skeletal muscle differentiation via IGF2 up-regulation.
• LIN28A is localized in the cytoplasm, particularly at the rough endoplasmic reticulum, and also found in P-bodies, stress granules, and the nucleolus.
• It is highly expressed in embryonic stem cells, placenta, and testis, and is often up-regulated in HER2-overexpressing breast tumors, linking it to cancer and pluripotency.
• The protein contains tandem zinc finger CCHC domains and undergoes key post-translational modifications including acetylation and phosphorylation, which regulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody generation spans amino acids 100-200 of human LIN28A, a region likely containing functional domains; consider this when designing validation experiments.
• For Western blot, LIN28A has a predicted molecular weight of ~23 kDa; optimize blocking and detection conditions to clearly resolve the band.
• For IHC-P, antigen retrieval may be necessary; validate antibody specificity in tissue samples with known LIN28A expression (e.g., testis or tumor sections).
• The antibody cross-reacts with mouse and rat; verify performance in your specific model organism to ensure consistent results.
• For ELISA, pair with a suitable detection antibody; titrate both capture and detection reagents for optimal sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering validated antibody pairs, optimized recombinant monoclonal antibodies, and bulk ancillary reagents to streamline your diagnostic development. Our anti-LIN28A antibody supports research into stem cell pluripotency, developmental biology, and oncology, with reliable performance across multiple applications. Whether you need this antibody or other rare-target raw materials, we provide end-to-end sourcing solutions to accelerate your project from concept to clinic.