Anti-LIMS1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P48059

Core Product Specifications and Parameters

Biological Background: LIMS1 Function and Localization

• LIMS1, also known as PINCH-1 or renal carcinoma antigen NY-REN-48, is a LIM domain-containing protein that acts as a key adaptor within the ILK-PINCH-PARVIN (IPP) complex.
• It directly binds to F-actin and promotes F-actin bundling, which generates the mechanical force required for actin cytoskeleton reorganization and dynamic cell adhesion events such as cell spreading and migration.
• The protein is an essential component of focal adhesions and localizes to cell junctions and the cell membrane, where it mediates cell–matrix interactions.
• LIMS1 is expressed in most human tissues but is notably absent or expressed at very low levels in the brain.
• The protein contains multiple LIM zinc-binding domains that facilitate protein–protein interactions, and it undergoes post-translational modifications including acetylation.
• Alternative splicing of the LIMS1 gene produces multiple isoforms, which may contribute to functional diversity in different cellular contexts.

Experimental Guidance and Technical Tips

• The immunogen used to generate this rabbit polyclonal antibody covers amino acids 1–325 of human LIMS1; therefore, it is expected to recognize multiple isoforms and may be suitable for detecting endogenous LIMS1 in human, mouse, and rat cell lysates.
• For Western blot (WB), a band at approximately 37 kDa is predicted. Optimization of sample preparation and antibody dilution is recommended to minimize non-specific binding.
• In immunofluorescence (IF/ICC), focal adhesion staining is anticipated; consider permeabilization conditions and appropriate fixation methods to preserve the actin cytoskeleton architecture.
• ELISA performance can be assessed using the recombinant immunogen as a positive control; validation in biologically relevant sample matrices is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges diagnostic manufacturers and research institutions with a comprehensive range of IVD raw materials. For LIMS1-related studies in focal adhesion and cell migration pathways, we offer validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents to streamline assay development. Whatever your sourcing needs—from rare-target antibodies to large-scale production—CamelBio is your reliable partner from concept to clinic.