Anti-LIMS1 Polyclonal Antibody for WB, IF/ICC, ELISA - P48059

Core Product Specifications and Parameters

Biological Background: LIMS1 Function and Localization

• LIMS1 (LIM and senescent cell antigen-like-containing domain protein 1), also known as PINCH-1 or particularly interesting new Cys-His protein 1, is a key component of the IPP (ILK-PINCH-PARVIN) complex that bridges integrins to the actin cytoskeleton.
• Through its interaction with ILK and parvin, LIMS1 binds to F‑actin and promotes F‑actin bundling, a process essential for generating mechanical force during actin cytoskeleton reorganization.
• This activity drives dynamic cell adhesion events, including cell spreading and migration, by facilitating focal adhesion turnover and maturation.
• LIMS1 localizes to cell junctions and focal adhesions at the plasma membrane, where it serves as a scaffold for signaling and structural proteins.
• It is broadly expressed across most human tissues, with notably low or absent expression in the brain.
• Structurally, LIMS1 contains five LIM domains that coordinate zinc ions and mediate protein–protein interactions; it is also subject to acetylation and alternative splicing.

Experimental Guidance and Technical Tips

• This polyclonal antibody, raised against the full-length human LIMS1 (aa 1–325), can be used in WB, IF/ICC, and ELISA. For WB, the predicted molecular weight is ~37 kDa; validate with appropriate positive controls (e.g., HeLa, MCF‑7 cell lysates) to confirm specificity.
• For IF/ICC, LIMS1 exhibits a focal adhesion-like staining pattern; consider optimizing fixation (e.g., 4% PFA) and permeabilization (0.1% Triton X‑100) to preserve antigenicity.
• As cross-reactivity is confirmed for human and mouse, this antibody is suitable for studies in widely used mouse cell lines (e.g., NIH/3T3) and tissues, but validation in the specific sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive procurement platform for IVD raw materials, serving diagnostic manufacturers, research laboratories, and clinical institutions worldwide. The anti-LIMS1 polyclonal antibody supports investigations into cell adhesion, cytoskeletal dynamics, and cancer biology. Beyond this product, CamelBio can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials, providing end-to-end support from concept to clinic.