Anti-LIMCH1 Polyclonal Antibody for WB, ELISA - Q9UPQ0

Core Product Specifications and Parameters

Biological Background: LIMCH1 Function and Localization

• LIMCH1 is a LIM and calponin homology domains-containing protein that associates with actin stress fibers and activates non-muscle myosin IIa.
• It promotes the phosphorylation of the myosin regulatory light chain (MRLC/MYL9), thereby enhancing the activity of the non-muscle myosin IIa complex.
• Through myosin IIa activation, LIMCH1 positively regulates the assembly of actin stress fibers and stabilizes focal adhesions, leading to reduced cell spreading and migration.
• The protein contains LIM domains, a coiled-coil region, and a calponin homology domain, and is classified as a metal-binding phosphoprotein.
• It is localized to the cytoplasm and specifically to the actin cytoskeleton at stress fibers.
• Alternative splicing of the LIMCH1 gene generates multiple transcript variants, although functional differences among isoforms remain to be fully characterized.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 150-450) of LIMCH1, which may provide specific detection in Western blot when antibody specificity is validated in relevant sample systems.
• For ELISA applications, consider using the antibody as a capture or detection reagent after confirming reactivity with the target epitope in your assay format.
• When performing WB, a band around 122 kDa is expected; use appropriate molecular weight markers and verify absence of non-specific bands in control lysates.
• Due to the role of LIMCH1 in stress fiber regulation, it may be informative to compare protein levels in cells treated with cytoskeleton-modifying agents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with seamless access to a broad portfolio of IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare-target biomolecules. This anti-LIMCH1 polyclonal antibody supports research into actin dynamics, cell migration, and adhesion pathways. Backed by robust supply chains and technical support, CamelBio helps accelerate your assay development from concept to clinic.