Anti-LIMA1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9UHB6

Core Product Specifications and Parameters

Biological Background: LIMA1 Function and Localization

• Encoded by the LIMA1 gene (also known as EPLIN), alternative splicing produces isoforms alpha and beta; isoform alpha is predominantly expressed in epithelial cells.
• Actin-binding protein that regulates cytoskeleton dynamics by increasing stress fiber number and size, inhibiting membrane ruffling, preventing actin filament depolymerization, and bundling filaments while delaying nucleation and reducing branched filaments. Related references: PMID:12566430 PMID:33999101
• Acts as a negative regulator of primary cilium formation. Related references: PMID:32496561
• Plays a role in cholesterol homeostasis by influencing plasma cholesterol levels through regulation of intestinal cholesterol absorption; may function as a scaffold protein that recruits MYO5B to NPC1L1 at the plasma membrane.
• Localizes to multiple subcellular structures including the cytoplasm, focal adhesions, stress fibers, cell membrane, ruffles, and lamellipodia.
• Highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen, and heart; also detected in lung, liver, brain, skeletal muscle, thymus, testis, and intestine but absent in leukocytes. Isoform alpha is abundant in epithelial cells from mammary gland and prostate.
• Post-translational modifications include acetylation and phosphorylation; contains a LIM domain and is classified as a metal-binding zinc protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 560–759 of human LIMA1, a region that may yield antibodies recognizing both isoforms; consider sequence alignment with the mouse ortholog to assess cross-reactivity.
• For Western blot, the expected molecular weight is approximately 85 kDa; validate band specificity using appropriate controls and lysates from human or mouse cells.
• In immunohistochemistry (paraffin), test on human epithelial tissues (e.g., breast, prostate) where LIMA1 alpha is highly expressed; antigen retrieval conditions may require optimization.
• The polyclonal nature provides detection of multiple epitopes, making it suitable for sensitive ELISA applications; pairing with a monoclonal capture antibody is recommended for sandwich ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this anti-LIMA1 polyclonal antibody to support research in cytoskeletal dynamics and cholesterol metabolism. As a dedicated IVD raw-material sourcing partner, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, helping diagnostic manufacturers and labs streamline assay development from concept to clinic.