Anti-LIM2 Rabbit Polyclonal Antibody for WB and ELISA - P55344

Core Product Specifications and Parameters

Biological Background: LIM2 Function and Localization

• LIM2 (also known as MP18/MP19/MP20) is a lens fiber membrane intrinsic protein encoded by the LIM2 gene (GeneID: 3982), with a predicted molecular weight of approximately 20 kDa.
• The protein is specifically expressed in the eye lens and is located in the plasma membrane of lens fiber cells.
• LIM2 is a major component of the thicker 16–17 nm junctions in mammalian lens fiber cells, where it contributes to cell junctional organization and lens transparency.
• It acts as a receptor for calmodulin, suggesting a role in calcium-mediated signaling within the lens.
• Functional studies indicate LIM2 may play an important role in both lens development and cataractogenesis; mutations in LIM2 are associated with congenital cataracts.
• Post‑translational modifications include glycosylation, phosphorylation, and disulfide bond formation.
• The protein contains transmembrane helices and is classified as a multi‑pass membrane protein.
• Alternative splicing of LIM2 produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 25–110) of human LIM2; this region is predicted to be extracellular or cytoplasmic, depending on the membrane topology model.
• For Western blot, expect a specific band around 20 kDa under reducing conditions; validate detection in lens tissue extracts or cell lines expressing LIM2.
• Membrane protein extraction may require detergent-based solubilization; consider optimizing lysis buffer to maintain antigen solubility.
• In ELISA, the antibody can be used for quantitative detection of LIM2 in lens-derived samples or recombinant protein standards.
• Cross‑reactivity with mouse and rat orthologs is indicated; sequence alignment of the immunogen region is recommended to confirm epitope conservation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this anti-LIM2 rabbit polyclonal antibody for lens biology and cataract research. As a one‑stop sourcing partner, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw materials—supporting diagnostic manufacturers and research labs from early development to clinical application.