Anti-LILRB3 Polyclonal Antibody for WB, ELISA - O75022

Core Product Specifications and Parameters

Biological Background: LILRB3 Function and Localization

• LILRB3, also known as ILT5, LIR3, and CD85a, is a member of the leukocyte immunoglobulin-like receptor (LILR) family.
• It functions as an inhibitory receptor, potentially recognizing class I MHC antigens, and upon co-ligation with immune receptors like FCGR2B, it down-regulates B-cell activation by recruiting phosphatases to its ITIM motifs.
• The gene undergoes alternative splicing, producing multiple isoforms.
• LILRB3 is a single-pass type I membrane protein localized to the cell membrane.
• Post-translational modifications include glycosylation and phosphorylation; it contains disulfide bonds and immunoglobulin-like domains, with its 3D structure determined.
• It is predominantly expressed in immune cells, specifically detected in monocytes and B-cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 400–500 of human LILRB3; consider this epitope location when designing detection strategies.
• For Western blot, the predicted molecular weight is approximately 69 kDa; validate in relevant cell lysates known to express LILRB3, such as B-cell or monocyte lines.
• In ELISA, optimize antibody concentration and consider using purified recombinant LILRB3 as a positive control.
• Cross-reactivity has been reported with human and mouse; verify reactivity in your specific sample system if not human.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to validated IVD raw materials, including a comprehensive portfolio of antibodies for immune checkpoint and receptor targets like LILRB3. Our one-stop sourcing model covers monoclonal and polyclonal antibody development, bulk reagent supply, and customized technical support to accelerate your projects.