Anti-LILRB2/CD85d/ILT4 Rabbit Polyclonal Antibody for WB/ELISA - Q8N423

Core Product Specifications and Parameters

Biological Background: LILRB2/CD85d/ILT4 Function and Localization

• LILRB2, also known as CD85d, ILT-4, LIR-2, and MIR-10, is a member of the leukocyte immunoglobulin-like receptor (LILR) family.
• It functions as a receptor for classical and non-classical MHC class I antigens, including HLA-A, HLA-B, HLA-C, HLA-G, and HLA-F, and is implicated in immune tolerance. Related references: PMID:11169396, PMID:12853576, PMID:16455647
• Binding of HLA-G in complex with beta-2 microglobulin and nonamer self-peptide triggers differentiation of regulatory T cells and myeloid-derived suppressor cells, crucial for maternal-fetal tolerance. Related references: PMID:16455647, PMID:20448110, PMID:27859042
• Competes with CD8A for MHC class I binding and negatively regulates FCGR1A-mediated phosphorylation and intracellular calcium mobilization. Related references: PMID:11875462, PMID:12853576, PMID:9548455
• Predominantly expressed on monocytes, with lower levels on dendritic cells, B cells, and NK cells; enriched in tolerogenic IL-10-producing dendritic cells and pregnancy-associated myeloid-derived suppressor cells. Related references: PMID:20448110, PMID:27859042
• Localizes to the cell membrane with a single-pass transmembrane domain and contains immunoglobulin-like domains characteristic of inhibitory receptors.
• Undergoes alternative splicing, alternative promoter usage, and alternative initiation, producing isoforms with distinct functional properties.
• Post-translational modifications include phosphorylation and glycosylation, which may regulate its signaling and surface expression.

Experimental Guidance and Technical Tips

• For Western blot, use reducing conditions and validate with positive controls such as LILRB2-expressing monocytic cell lines (e.g., THP-1) and negative controls (e.g., LILRB2-knockdown lysates).
• This antibody targets an intracellular epitope (aa 479-598); it is not suitable for live-cell flow cytometry without permeabilization.
• For ELISA, consider pairing with a capture antibody if developing a sandwich immunoassay, and optimize antigen coating and detection conditions.
• Inclusion of protease and phosphatase inhibitors in lysis buffers is recommended to preserve protein integrity and phosphorylation status.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing diagnostic manufacturers and researchers with validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like LILRB2, which plays a critical role in immune regulation and tolerance pathways, CamelBio can assist with rare target raw-material sourcing and custom solutions to accelerate your diagnostic development. Contact us to explore how our one-stop services can support your project from concept to clinic.