Anti-LILRA2/CD85h Rabbit Monoclonal Antibody for IF/ICC, FC, ELISA - Q8N149

Core Product Specifications and Parameters

Biological Background: LILRA2/CD85h Function and Localization

• LILRA2 (also known as ILT1, LIR7, CD85H) is a leukocyte immunoglobulin-like receptor encoded by the LILRA2 gene in humans. It belongs to the LILR family and has multiple isoforms due to alternative splicing (predicted molecular weights: 53, 51, 48, 47 kDa).
• LILRA2 functions as an innate immune receptor that specifically recognizes N-terminally truncated immunoglobulins produced by bacterial and fungal proteases, thereby mediating host defense against pathogens such as L. pneumophila, S. aureus, and C. albicans. Related references: PMID:27572839
• Upon ligand binding, LILRA2 activates neutrophils and triggers the release of pro-inflammatory cytokines (e.g., IL6, CXCL8) from monocytes, while downregulating TLR4-mediated responses to LPS. Related references: PMID:12529506 PMID:22479404
• In eosinophils, LILRA2 stimulation leads to degranulation and secretion of RNASE2, IL4, and leukotriene C4. Related references: PMID:12529506
• LILRA2 does not bind classical MHC class I molecules, distinguishing it from other LILR family members. Related references: PMID:19230061
• The receptor is primarily expressed on the cell membrane of peripheral blood monocytes, neutrophils, basophils, and eosinophils, but is absent or very low on lymphocytes and NK cells. A secreted isoform may also exist. Related references: PMID:12529506 PMID:22479404 PMID:9548455
• LILRA2 is a glycoprotein with disulfide bond-stabilized immunoglobulin-like domains; it undergoes post-translational nitration.

Experimental Guidance and Technical Tips

• Given that the immunogen corresponds to the extracellular domain (aa 24-449), this antibody may be suitable for detecting native surface-expressed LILRA2 in flow cytometry (FC) and immunofluorescence/immunocytochemistry (IF/ICC).
• For ELISA, consider using recombinant LILRA2 protein as a standard and validate in the appropriate assay format.
• Optimal working concentrations should be determined empirically; perform titration experiments in your specific sample system.
• Confirm antibody specificity using LILRA2-positive cells such as primary monocytes or neutrophils, and use LILRA2-negative lymphocytes as a negative control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable, one-stop access to IVD raw materials for innate immunity and receptor biology. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to accelerate assay development. Whether you need antibodies targeting LILRA2 for microbial infection research or rare target sourcing, CamelBio bridges the gap from concept to clinic.