Anti-LILRA2/CD85h Monoclonal Antibody for IF/ICC, FC - Q8N149

Core Product Specifications and Parameters

Biological Background: LILRA2 Function and Localization

• LILRA2 (Leukocyte immunoglobulin-like receptor subfamily A member 2), also known as ILT-1, LIR-7, CD85H, is a type I transmembrane receptor belonging to the leukocyte immunoglobulin-like receptor family.
• Key innate immune receptor that specifically recognizes N-terminally truncated immunoglobulins generated by proteases from pathogenic bacteria and fungi (e.g., L. pneumophila, S. pneumoniae, S. aureus, C. albicans) to mediate host defense. Related references: PMID:27572839
• Binding of cleaved immunoglobulins activates neutrophils, contributing to antimicrobial responses. Related references: PMID:27572839
• In monocytes, ligand engagement induces release of pro-inflammatory cytokines (CSF2, CF3, IL6, CXCL8, CCL3) and downregulates TLR4-mediated responses to LPS. Related references: PMID:22479404
• In eosinophils, activation triggers degranulation and secretion of RNASE2, IL4, and leukotriene C4, promoting allergic/inflammatory reactions. Related references: PMID:12529506
• Unlike other LILR family members, LILRA2 does not bind classical MHC class I molecules. Related references: PMID:19230061
• Subcellular localization: predominantly expressed on the cell membrane; also found as a secreted form.
• Tissue distribution: highly expressed on peripheral blood monocytes, neutrophils, basophils, and eosinophils; minimal on T-cells, B-cells, dendritic cells, and NK cells. Related references: PMID:12529506 PMID:22479404 PMID:9548455
• Listed keywords: alternative splicing (generating isoforms of ~48-53 kDa), glycoprotein, disulfide bond, immunoglobulin domain, innate immunity receptor.

Experimental Guidance and Technical Tips

• For immunofluorescence (IF/ICC), test different fixation and permeabilization conditions to optimize detection of this membrane and secreted receptor.
• In flow cytometry (FC), titrate the antibody on relevant primary human cells (monocytes, neutrophils) to determine optimal concentration.
• The antibody is directly conjugated to , eliminating the need for a secondary antibody; use appropriate filter sets for green fluorescence detection.
• Note that the immunogen spans the extracellular domain (aa 24-449); multiple protein weights (47–53 kDa) correspond to reported isoforms from alternative splicing.
• Always include proper isotype controls and validate specificity in the biological context of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and researchers with validated antibody pairs, recombinant proteins, and ancillary reagents. This anti-LILRA2 antibody supports immunoassay development in innate immunity, autoimmune, and infectious disease research. CamelBio's streamlined supply chain ensures rapid access to rare-target reagents, accelerating your progress from concept to clinic.