Anti-LHX9 Rabbit Polyclonal Antibody for WB, ELISA - Q9NQ69

Core Product Specifications and Parameters

Biological Background: LHX9 Function and Localization

• LHX9 (LIM/homeobox protein Lhx9) is a member of the LIM-homeodomain family of transcription factors, characterized by two N-terminal LIM domains and a C-terminal homeobox DNA-binding domain.
• It plays a crucial role in gonadal development, functioning as a transcription factor that regulates target gene expression.
• LHX9 localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• The LIM domains coordinate zinc ions, facilitating protein-protein interactions and DNA-binding specificity.
• Alternative splicing generates multiple isoforms of LHX9, which may have distinct functional properties.
• Keywords associated with LHX9 include DNA-binding, Homeobox, LIM domain, Metal-binding, Nucleus, and Zinc, reflecting its molecular nature.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-70, covering the N-terminal region. This antibody may recognize multiple LHX9 isoforms that share this epitope.
• For Western blot (WB), a predominant band at approximately 44 kDa is anticipated, consistent with the calculated molecular weight; however, alternative splicing products may appear. Validate specificity using appropriate controls.
• In ELISA, optimize coating antigen concentration and antibody dilution to achieve optimal signal-to-noise ratio; cross-reactivity with mouse and rat enables multi-species studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, supporting projects from early development to clinical application. For researchers focusing on reproductive biology and transcription factor studies, we provide access to validated antibodies like this LHX9 polyclonal antibody, along with bulk reagents and custom sourcing for rare targets.