Anti-LHX8 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q68G74

Core Product Specifications and Parameters

Biological Background: LHX8 Function and Localization

• LHX8 (also known as LHX7) is a LIM-homeobox transcription factor that contains both LIM domains for protein interactions and a homeobox domain for DNA binding.
• It is essential for the differentiation of specific neuronal populations and mesenchymal cells during embryonic development.
• LHX8 is localized to the nucleus, where it regulates gene expression.
• The gene undergoes alternative splicing, producing multiple isoforms (UniProt keyword: Alternative splicing).
• The LIM domains coordinate zinc ions, classifying LHX8 as a metal-binding protein (UniProt keywords: Metal-binding, Zinc).
• As a transcription regulator, LHX8 is involved in developmental processes and cell fate determination (UniProt keywords: Transcription, Transcription regulation, Developmental protein).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–220, covering the N-terminal region that likely includes the LIM domains, which may allow recognition of conserved epitopes across isoforms.
• For Western blot (WB), consider using lysates from human neuronal cell lines or tissues rich in LHX8 expression to optimize detection; the predicted molecular weight is approximately 39 kDa.
• In immunofluorescence/ICC, expect nuclear staining; counterstaining with DAPI is recommended to confirm localization.
• For ELISA, validate the antibody’s reactivity with the recombinant protein or peptide to establish a standard curve; consider testing with native samples if applicable.
• As a polyclonal antibody, it may exhibit higher sensitivity but potential batch-to-batch variation; include appropriate controls in your experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a comprehensive sourcing partner for IVD raw materials, offering everything from validated antibody pairs to bulk ancillary reagents. This anti-LHX8 polyclonal antibody supports neuroscience and developmental biology research, and CamelBio can assist with procuring rare-target reagents and scaling up for diagnostic assay development. Whether you need monoclonal or polyclonal antibodies, recombinant proteins, or technical consulting, CamelBio provides a streamlined pathway from concept to clinic.