Anti-LHX6 Rabbit Polyclonal Antibody for WB, ELISA - Q9UPM6

Core Product Specifications and Parameters

Biological Background: LHX6 Function and Localization

• LHX6 (LIM/homeobox protein Lhx6), also known as LHX6.1, is a member of the LIM-homeodomain transcription factor family.
• It functions as a probable transcription factor essential for the expression of genes involved in interneuron migration and development, particularly in the specification of cortical interneuron subtypes and the migration of GABAergic interneuron precursors from the subpallium to the cerebral cortex.
• The protein contains classical LIM domains and a homeobox DNA-binding domain, allowing both protein-protein interactions and sequence-specific DNA binding.
• Subcellularly, LHX6 is localized to the nucleus, consistent with its role in transcriptional regulation.
• Key post-translational modifications and functional features include metal-binding (zinc) via LIM domains, and involvement in developmental processes, neurogenesis, and differentiation.
• The gene undergoes alternative splicing, producing multiple isoforms with observed molecular weights ranging from 19 kDa to 43 kDa as detected in various assays.
• LHX6 is part of the proteomics identification reference proteome, underscoring its relevance in high-throughput studies.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 250-350 of human LHX6 (NP_001229263.1), which may recognize multiple isoforms. Validate detection of all expected isoforms in your target tissue or cell line.
• For Western blot (WB), consider using positive control lysates from tissues or cells with known LHX6 expression, such as developing brain or neuronal cell cultures. Expected molecular weights may vary between 19–43 kDa due to alternative splicing.
• For ELISA, validate the antibody with recombinant LHX6 protein or the immunizing peptide to confirm specificity and establish a standard curve.
• As a polyclonal antibody, lot-to-lot variability can occur; perform titration experiments to determine the optimal working concentration for your specific assay.
• Ensure proper negative controls (e.g., lysates from LHX6 knockdown or knockout cells) to confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, labs, and research institutes with end-to-end access to IVD raw materials and technical support. For LHX6-related neurodevelopmental studies, consider our extended portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. From concept to clinic, CamelBio is your reliable partner in accelerating assay development.